Coding

Part:BBa_K4041011:Design

Designed by: Derek Zhang   Group: iGEM21_HKIS   (2021-10-21)


pET-LO-SUMO-AMP-Tachy Original


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 560
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 370
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutation of EcoRI and PstI to match the RFC10 standard.


Source

Cloned

References